Tutorials

📄️ Correcting baseline with topHat

There are moments when an image doesn't have a perfect lightning and you have uneven background like here.

📄️ Extracting metadata

In this tutorial we will discuss metadata extraction with an image that we already familiar with: the image of particles. We will also talk about pixel size and the way it is calculated.

📄️ Image segmentation with threshold

In this tutorial we are going to cover the threshold operation and how to get a map of regions from the threshold's mask.

📄️ Image segmentation with watershed

In this tutorial we will talk about watershed algorithm, why it is used and the basics of how it is implemented. We will also cover some pitfalls that you might encounter during its use.

📄️ Image stack analysis

The point of this tutorial is to show how to decode a stack of images and how to do some basic analysis with it.

📄️ ROI analysis

Before taking on the analysis of regions of interest we recommend to take a look at the tutorials for watershed and threshold, notably their options and the blurring techniques to optimize the output.